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NEDD9 antibody (AA 82-398)

NEDD9 Reactivity: Human, Rat, Mouse WB, IF, IP, ICC, FACS, IHC (fro) Host: Mouse Monoclonal 2G9 unconjugated
Catalog No. ABIN7278755
  • Target See all NEDD9 Antibodies
    NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
    Binding Specificity
    • 9
    • 9
    • 7
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 82-398
    Reactivity
    • 46
    • 11
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 39
    • 6
    • 1
    Mouse
    Clonality
    • 41
    • 5
    Monoclonal
    Conjugate
    • 24
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This NEDD9 antibody is un-conjugated
    Application
    • 26
    • 18
    • 14
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Does not recognize p130 Cas. Not tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites
    Purification
    Protein G
    Immunogen
    Fusion protein, corresponding to amino acids 82-398 of Human HEF1.
    Clone
    2G9
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product NEDD9 Primary Antibody
  • Application Notes
    Optimal antibody dilution should be determined by titration, however as a guideline try, WB: 1/2000 - 1/6000. Predicted molecular weight: 93 kDa. IP: 1/1000. ICC/IF: Use at an assay dependent dilution. PubMed: 19376971 IHC-Fr: Use at an assay dependent dilution. PubMed: 19464348 Flow Cyt: Use 1 μg for 106 cells
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Purified antibody containing PBS 0.1 % sodium azide,
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Tomkiewicz, Herry, Bui, Métayer, Bourdeloux, Barouki, Coumoul: "The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathway." in: Oncogene, Vol. 32, Issue 14, pp. 1811-20, (2013) (PubMed).

    Zhong, Baquiran, Bonakdar, Lees, Ching, Pugacheva, Fabry, ONeill: "NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration." in: PLoS ONE, Vol. 7, Issue 4, pp. e35058, (2012) (PubMed).

    ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

  • Target
    NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
    Alternative Name
    HEF1 (NEDD9 Products)
    Synonyms
    NEDD9 antibody, CAS-L antibody, CAS2 antibody, CASL antibody, CASS2 antibody, HEF1 antibody, Cas-L antibody, CasL antibody, E230025G09Rik antibody, neural precursor cell expressed, developmentally down-regulated 9 antibody, neural precursor cell expressed, developmentally down-regulated gene 9 antibody, NEDD9 antibody, Nedd9 antibody
    Background
    Synonyms:
    CAS L antibody,Cas like docking antibody,CASL antibody,Crk associated substrate related proteinantibody,dJ49G10.2 antibody,dJ761I2.1 antibody,Enhancer of filamentation 1 antibody,HEF 1 antibody,NEDD 9antibody,NEDD9 antibody,NEDD9 protein antibody,Neural cell expressed developmentally down regulated 9antibody,Neural precursor cell expressed developmentally down regulated 9 antibody,NY REN 12 antigenantibody,p105 antibody,Protein NEDD9 antibody
    Gene ID
    4739, 18003, 291044
    OMIM
    602265
    UniProt
    O35177
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