CD66acde antibody
CEACAM1/3/5/6
Reactivity: Human
FACS, IF, IP, ELISA, WB, cELISA, IHC (fro)
Host: Mouse
Monoclonal
D14HD11
unconjugated
-
- Target
- CD66acde (CEACAM1/3/5/6) (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/3/5/6 (CEACAM1/3/5/6))
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB), Cell-ELISA (cELISA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Anti-human CEACAM1,3,4,5,6 (CD66a,c,d,e,CGM7)
- Purification
- Protein G
- Immunogen
- immunisation with extracted protein of CEACAM5
- Clone
- D14HD11
- Isotype
- IgG1
-
- Application Notes
-
Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400 Western Blot: 4 µg/mL
Immunofluorescence: 1 µg/106cells
For each application a titration should be performed to determine the optimal concentration. - Comment
-
Synonyms: CD66acde, CGM7
- Restrictions
- For Research Use only
-
- Buffer
- PBS, pH 7.2
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C
- Storage Comment
- short term: 2 °C - 8 °C, long term: -20 °C
-
- Target
- CD66acde (CEACAM1/3/5/6) (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/3/5/6 (CEACAM1/3/5/6))
- Alternative Name
- CEACAM1,3,4,5,6
- Background
- D14HD11 was generated by immunisation with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors. D14HD11 is a broadly reactive high affinity antibody which reacts with most of the CEACAM molecules (except CEACAM7 and CEACAM8).
- UniProt
- P06731, O75871, P13688, P40199, P40198
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