ANK2 antibody (AA 203-496)

Catalog No. ABIN1098194

Product Details anti-ANK2 Antibody

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Binding Specificity: AA 203-496

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-Ankyrin-B Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Ankyrin-B.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Synthetic peptide correspond- ing to aa 203-496 of human Ankyrin-B (accession no. NP_001120965.1)

Clone: S105-13

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of >200 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details for ANK2

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Alternative Name: Ankyrin-B (ANK2 Products)

Synonyms: ANK-2 antibody, LQT4 antibody, brank-2 antibody, AI835472 antibody, AW491075 antibody, Ank-2 antibody, Ank3 antibody, CHANK2 antibody, ANK2 antibody, ank2 antibody, zgc:101738 antibody, ANKB antibody, ankyrin 2 antibody, ankyrin 2, brain antibody, ankyrin-2 antibody, ankyrin 2b, neuronal antibody, ANK2 antibody, Ank2 antibody, LOC539238 antibody, ank2b antibody, LOC100465763 antibody

Background: Ankyrin-2,Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity). {UniProtKB:Q8C8R3, PubMed:12571597}.,Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.,Cytoplasm, cytoskeleton, Membrane, Cytoplasm, myofibril, sarcomere, M line, Apical cell membrane, Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Early endosome, Recycling endosome, Lysosome, Mitochondrion, Cytoplasm, myofibril, sarcomere, Z line, Cell membrane, sarcolemma, T-tubule,ANK-2, Ankyrin-B, Brain ankyrin, Non-erythroid ankyrin

UniProt: Q01484

Pathways: Cell-Cell Junction Organization