SLC1A1
Reactivity: Human
WB, IP, ICC, IF
Host: Mouse
Monoclonal
S180-41
Atto 488
Application Notes
Immunoblotting: use at 1-5 μg/mL. A band of ~60 kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Dilute in PBS or medium that is identical to that used in the assay system.
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Target
SLC1A1
(Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))
Excitatory amino acid transporter 3,Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7914198, PubMed:7521911, PubMed:8857541, PubMed:26690923, PubMed:21123949). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:7521911, PubMed:8857541, PubMed:26690923). Mediates Cl(-) flux that is not coupled to amino acid transport, this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:8857541, PubMed:26690923). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity). {UniProtKB:P51906, UniProtKB:P51907, PubMed:21123949, PubMed:26690923, PubMed:7521911, PubMed:7914198, PubMed:8857541}.,Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L- glutamate/D,L-aspartate cell membrane transport protein. It is expressed in brain as well as in several non-nervous system tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and functions mainly perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic amino acid reabsorption and amino acid metabolism. EAAC1- associated protein GTRAP3-18 may be important in regulating the metabolic function of EAAC1.,Cell membrane, Apical cell membrane, Cell junction, synapse, synaptosome, Early endosome membrane, Late endosome membrane, Recycling endosome membrane,Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1