KLHDC8B antibody (AA 259-270)

Catalog No. ABIN1102756

Product Details anti-KLHDC8B Antibody

Target: KLHDC8B (Kelch Domain Containing 8B (KLHDC8B))

Binding Specificity: AA 259-270

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This KLHDC8B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human KLHDC8B.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Hamster, Panda, Rabbit (100%) Elephant, Horse, Porcine, Opossum, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TKLPRSLRMRDK from an internal region of human KLHDC8B (NP_775817.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Hamster, Panda, Rabbit (100%), Elephant, Horse, Porcine, Opossum, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:64000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kD band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 37.7kD according to NP_775817.1). Recommended concentration: 0.5-2 μg/mL. An additional band of unknown identity was also consistently observed at 22kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KLHDC8B

Target: KLHDC8B (Kelch Domain Containing 8B (KLHDC8B))

Alternative Name: KLHDC8B (KLHDC8B Products)

Synonyms: DKFZp468J2023 antibody, 4931406O17Rik antibody, kelch domain containing 8B antibody, KLHDC8B antibody, Klhdc8b antibody

Background: Name/Gene ID: KLHDC8B

Synonyms: KLHDC8B, Kelch domain containing 8B

Gene ID: 200942

NCBI Accession: NP_775817

UniProt: Q8IXV7