SIM1 antibody (AA 545-556)

Catalog No. ABIN1104289

Product Details anti-SIM1 Antibody

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Binding Specificity: AA 545-556

Reactivity: Human, Horse, Rabbit, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This SIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human SIM1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Bat, Panda, Horse, Rabbit, Porcine (100%) Mouse, Dog, Bovine, Hamster, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (92%) Rat, Elephant, Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SGDRYRTEQYQS from an internal region of human SIM1 (NP_005059.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Bat, Panda, Horse, Rabbit, Porcine (100%), Mouse, Dog, Bovine, Hamster, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (92%), Rat, Elephant, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:2000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 80kD band observed in lysates of cell line HEK293 (calculated MW of 85.5kD according to NP_005059.2). Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SIM1

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Alternative Name: SIM1 (SIM1 Products)

Synonyms: bHLHe14 antibody, mSIM1 antibody, single-minded family bHLH transcription factor 1 antibody, single-minded homolog 1 (Drosophila) antibody, single-minded family bHLH transcription factor 1a antibody, SIM1 antibody, Sim1 antibody, sim1a antibody

Background: Name/Gene ID: SIM1

Synonyms: SIM1, BHLHe14, Single-minded homolog 1

Gene ID: 6492

NCBI Accession: NP_005059

UniProt: P81133