TNFSF9 antibody

Catalog No. ABIN1104740

Product Details anti-TNFSF9 Antibody

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human 4-1BBL

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human 4-1BBL.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7425 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: Data Not Available. ELISA: To detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human 4-1BBL (LS-C104681) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBL. Western Blot: To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant h4-1BBL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFSF9

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: TNFSF9 / CD137L (TNFSF9 Products)

Synonyms: TNFSF9 antibody, 4-1BB-L antibody, 4-1BBL antibody, AI848817 antibody, Cd137l antibody, Ly63l antibody, CD137L antibody, TNF superfamily member 9 antibody, tumor necrosis factor (ligand) superfamily, member 9 antibody, Tnfsf9 antibody, LOC476729 antibody, TNFSF9 antibody

Background: Name/Gene ID: TNFSF9
Family: TNF

Synonyms: TNFSF9, 4-1BBL, 4-1BB ligand, 4-1BB-L, CD137L, Homolog of mouse 4-1BB-L, Receptor 4-1BB ligand

Gene ID: 8744

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints