ATP5J antibody (Middle Region)

Catalog No. ABIN1105468

Product Details anti-ATP5J Antibody

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects ATPase subunit F6 (mid region).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide mapping at the middle region of human mature ATP5J (CF6) .Remarks: Sequence is different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: ATPase Subunit F6 (ATP5J Products)

Synonyms: zgc:77541 antibody, wu:fa66e04 antibody, ATP5 antibody, ATP5A antibody, ATPM antibody, CF6 antibody, F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F antibody, atp5j antibody, ATP5J antibody, atp5j.L antibody, Atp5j antibody

Background: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72 % sequence identity with rat ATP5J.This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.Synonyms: ATP synthase, ATP synthase-coupling factor 6 mitochondrial, ATP5A, ATP5J, ATPM, Complex V subunit F6, F1F0 ATPase subunit F6, H+ transporting, mitochondrial F0 complex, subunit F6

Gene ID: 522

NCBI Accession: NP_001003696

UniProt: P18859

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process