PTPRJ antibody (N-Term)

Catalog No. ABIN1105887

Product Details anti-PTPRJ Antibody

Target: PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))

Binding Specificity: AA 1-444, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTPRJ antibody is un-conjugated

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Human recombinant CD148 (amino acids 1-444)

Clone: MEM-CD148-05

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), approx. pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PTPRJ

Target: PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))

Alternative Name: CD148 / PTPRJ (PTPRJ Products)

Synonyms: HAAP antibody, CD148 antibody, DEP1 antibody, HPTPeta antibody, R-PTP-ETA antibody, SCC1 antibody, AI450271 antibody, BET antibody, Byp antibody, DEP-1 antibody, PTPbeta2 antibody, Ptpb2 antibody, RPTPJ antibody, Scc-1 antibody, Scc1 antibody, PTPRJ antibody, acid phosphatase 1 antibody, protein tyrosine phosphatase, receptor type J antibody, protein tyrosine phosphatase, receptor type, J antibody, ACP1 antibody, PTPRJ antibody, Ptprj antibody, EUBREC_2762 antibody

Background: CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.Synonyms: DEP-1, DEP1, HPTP eta, Protein-tyrosine phosphatase eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J, R-PTP-eta, Receptor-type tyrosine-protein phosphatase eta

Gene ID: 5795

NCBI Accession: NP_002834

UniProt: Q12913

Pathways: EGFR Signaling Pathway, Platelet-derived growth Factor Receptor Signaling