POLI antibody (Middle Region, N-Term)

Catalog No. ABIN1107000

Product Details anti-POLI Antibody

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Binding Specificity: Middle Region, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Immunoaffinity column

Immunogen: Synthetic peptide directed towards the N-terminal to middle region of human POLI

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C for one month or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: DNA Polymerase iota (POLI Products)

Synonyms: poli antibody, wu:fc27h11 antibody, zgc:110185 antibody, GB12093 antibody, NV16187 antibody, RAD30B antibody, RAD3OB antibody, Rad30b antibody, DNA polymerase iota antibody, polymerase (DNA directed) iota antibody, polymerase (DNA directed), iota antibody, POLI antibody, poli antibody, LOC551219 antibody, AFUA_4G11140 antibody, LOC5568276 antibody, Pol-iota antibody, Poli antibody

Background: DNA polymerase iota (POLI) is an error-prone DNA polymerase specifically involved in DNA repair. POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. POLI favors Hoogsteen base-pairing in the active site. POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. POLI may play a role in hypermutation of immunogobulin genes. POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.Synonyms: Eta2, POLI, RAD30 homolog B, RAD30B

Molecular Weight: POLI: 83 kDa

Gene ID: 11201

NCBI Accession: NM_007195

Pathways: DNA Damage Repair