Podocalyxin-Like (PODXL) antibody (Biotin)

Details for Product No. ABIN1108686, Supplier: Log in to see
Antigen
  • PODXL
  • podxl
  • fc18d02
  • wu:fc18d02
  • Gp200
  • PC
  • PCLP
  • PCLP-1
  • AW121214
  • Ly102
  • Pclp1
  • podocalyxin
  • Gp135
  • PCLP1
  • cPCLP1
  • MEP21
  • Podxl
  • podocalyxin like
  • podocalyxin-like
  • uncharacterized LOC100614602
  • PODXL
  • podxl
  • LOC100614602
  • Podxl
Alternatives
anti-Human Podocalyxin-Like antibody for Immunocytochemistry
Reactivity
Mouse (Murine)
315
96
85
61
2
2
2
1
1
Host
Rat
190
123
23
20
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
20
19
16
15
12
12
10
9
9
8
7
7
6
5
5
5
4
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS)
218
186
99
96
90
74
56
41
36
10
5
5
3
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen LO cells
Clone 10B9
Isotype IgG1
Specificity This antibody detects Podocalyxin/PCLP1.
Cross-Reactivity (Details) Species reactivity (tested):Mouse
Purification Protein G agarose
Alternative Name Podocalyxin / PODXL (PODXL Antibody Abstract)
Background Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Anti-Mouse PCLP1 stains LO cells as well as the endothelial-like cells in the AGM culture. LO cell is a novel OSM (oncostatin M)-dependent cell line which exhibits characteristics similar to endothelial-like cells. Today it is reported that the PCLP1 is identical with the Podocalyxin.Synonyms: GCTM-2 antigen, Hematopoietic Stem Cell Marker, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1
Gene ID 27205
NCBI Accession NP_038751
UniProt Q9R0M4
Pathways Tube Formation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 50 µg/mL
Buffer PBS, 0.09 % NaN3, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Supplier Images
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (Biotin) (ABIN1108686) anti-Podocalyxin-Like (PODXL) antibody (Biotin)
Background publications Doyonnas, Nielsen, Chelliah, Drew, Hara, Miyajima, McNagny: "Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells." in: Blood, Vol. 105, Issue 11, pp. 4170-8, 2005 (PubMed).

Minegishi, Suzuki, Yokomizo, Pan, Fujimoto, Takahashi, Hara, Miyajima, Nishikawa, Yamamoto: "Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos." in: Blood, Vol. 102, Issue 3, pp. 896-905, 2003 (PubMed).

Minehata, Mukouyama, Sekiguchi, Hara, Miyajima: "Macrophage colony stimulating factor modulates the development of hematopoiesis by stimulating the differentiation of endothelial cells in the AGM region." in: Blood, Vol. 99, Issue 7, pp. 2360-8, 2002 (PubMed).

Hara, Nakano, Tanaka, Tamura, Sekiguchi, Minehata, Copeland, Jenkins, Okabe, Kogo, Mukouyama, Miyajima: "Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region." in: Immunity, Vol. 11, Issue 5, pp. 567-78, 2000 (PubMed).

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