RFWD2 antibody (Middle Region)

Catalog No. ABIN1108875

Product Details anti-RFWD2 Antibody

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse.

Purification: Immunoaffinity column

Immunogen: Synthetic peptide corresponding to a middle region of mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C for one month or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Target Details for RFWD2

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Alternative Name: RFWD2 / RNF200 (RFWD2 Products)

Synonyms: COP1 antibody, RNF200 antibody, AI316802 antibody, C80879 antibody, Cop1 antibody, RGD1304773 antibody, RFWD2 antibody, cop1 antibody, rnf200 antibody, zgc:163067 antibody, ring finger and WD repeat domain 2 antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog antibody, RFWD2 antibody, Rfwd2 antibody, rfwd2 antibody, rfwd2.L antibody

Background: Rfwd2 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It is involved in JUN ubiquitination and degradation and directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Rfwd2 probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.Synonyms: COP1, Constitutive photomorphogenesis protein 1 homolog, E3 ubiquitin-protein ligase RFWD2, RING finger and WD repeat domain protein 2, RING finger protein 200

Molecular Weight: RFWD2: 80 kDa

Gene ID: 26374

NCBI Accession: NM_011931

Pathways: Photoperiodism