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TJP1 antibody (AA 886-995)

TJP1 Reactivity: Human, Mouse, Rat, Dog WB, IF, FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1109613
  • Target See all TJP1 Antibodies
    TJP1 (Tight Junction Protein 1 (TJP1))
    Binding Specificity
    • 16
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 886-995
    Reactivity
    • 52
    • 31
    • 29
    • 20
    • 18
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog
    Host
    • 48
    • 3
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 50
    • 5
    Polyclonal
    Conjugate
    • 37
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TJP1 antibody is un-conjugated
    Application
    • 50
    • 19
    • 18
    • 15
    • 14
    • 13
    • 11
    • 11
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody recognizes specifically ZO-1 alpha-plus.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Mouse, Rat, Dog.
    Purification
    Protein A Chromatography
    Immunogen
    Amino acids 886-995 of human ZO-1
    Isotype
    Ig Fraction
    Top Product
    Discover our top product TJP1 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % Sodium Azide, 0.1 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 2-8 °C.
  • Target
    TJP1 (Tight Junction Protein 1 (TJP1))
    Alternative Name
    ZO-1 / Tight Junction Protein 1 (TJP1 Products)
    Synonyms
    ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody
    Background
    Zona occludens 1 (ZO-1) is an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1
    Gene ID
    7082
    NCBI Accession
    NP_003248
    UniProt
    Q07157
    Pathways
    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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