TUBB3 antibody (C-Term)

Catalog No. ABIN110980

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Binding Specificity: C-Term

Reactivity: Vicia faba

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Purified

Immunogen: Synthetic C-terminal peptide

Clone: TU-20

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 5-10 μg/mL (1/40-1/80). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/20). Proteinase K treatment forantigen retrieval is recommended. Recommended Positive Control: Human cortex. Has been described to work in ELISA and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer’s hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-TUBB3 antibody (ABIN110980)

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3 / TUBB4 (TUBB3 Products)

Synonyms: CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody

Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10 % of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III

Gene ID: 10381

NCBI Accession: NP_001184110

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase