alpha Fetoprotein antibody

Catalog No. ABIN111085

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in RIA and EIA. It exhibits reactivity toward Human.

Quick Overview for alpha Fetoprotein antibody (ABIN111085)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: 1301

Product Details anti-alpha Fetoprotein Antibody

Specificity: This antibody reacts with Human alpha Fetoprotein. No cross reactivity with Human albumin, CEA, Ferritin, hCG, PSA.Aff. Const.:5 x 10 e10

Purification: Protein A chromatography.

Immunogen: Purified AFP from Cord Blood

Isotype: IgG1

Application Details

Application Notes: For Enzyme conjugation, Radio-labeling or Solid Phase Immobilization. Suitable as detection antibody for Immunoassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 3.02 mg/mL (OD280 nm, E1% = 14)

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Background: Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

NCBI Accession: NP_001125

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process