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CD34 antibody (APC)

CD34 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal 581 APC
Catalog No. ABIN1111107
  • Target See all CD34 Antibodies
    CD34
    Reactivity
    • 300
    • 105
    • 104
    • 33
    • 19
    • 17
    • 17
    • 17
    • 16
    • 16
    • 9
    • 6
    • 5
    • 1
    • 1
    Human, Non-Human Primate
    Host
    • 169
    • 145
    • 17
    • 4
    Mouse
    Clonality
    • 216
    • 116
    • 2
    Monoclonal
    Conjugate
    • 128
    • 28
    • 24
    • 21
    • 13
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD34 antibody is conjugated to APC
    Application
    • 233
    • 203
    • 90
    • 64
    • 58
    • 53
    • 39
    • 37
    • 30
    • 28
    • 24
    • 20
    • 13
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
    No Cross-Reactivity
    Sheep
    Cross-Reactivity (Details)
    Human, Non-Human Primates
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Clone
    581
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD34 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

    Sanz, Muñoz-A, Monserrat, Van-Den-Rym, Escoll, Ranz, Alvarez-Mon, de-la-Hera: "Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 13, pp. 5925-30, (2010) (PubMed).

    Goardon, Nikolousis, Sternberg, Chu, Craddock, Richardson, Benson, Drayson, Standen, Vyas, Freeman: "Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes." in: Haematologica, Vol. 94, Issue 8, pp. 1160-3, (2009) (PubMed).

    Ninos, Jefferies, Cogle, Kerr: "The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells." in: Journal of translational medicine, Vol. 4, pp. 9, (2006) (PubMed).

    Ono, Sharma, Smith, Burnett, Aurelian: "CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM)." in: The Journal of investigative dermatology, Vol. 124, Issue 6, pp. 1215-24, (2005) (PubMed).

    Kato, Ando, Nakamura, Muguruma, Sato, Yabe, Yabe, Hattori, Yasuda, Hotta: "Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation." in: Bone marrow transplantation, Vol. 28, Issue 6, pp. 587-95, (2001) (PubMed).

    Felschow, McVeigh, Hoehn, Civin, Fackler: "The adapter protein CrkL associates with CD34." in: Blood, Vol. 97, Issue 12, pp. 3768-75, (2001) (PubMed).

    Ando, Nakamura, Chargui, Matsuzawa, Tsuji, Kato, Hotta: "Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system." in: Experimental hematology, Vol. 28, Issue 6, pp. 690-9, (2000) (PubMed).

    Fluckiger, Sanz, Garcia-Lloret, Su, Hao, Kato, Quan, de la Hera, Crooks, Witte, Rawlings: "In vitro reconstitution of human B-cell ontogeny: from CD34(+) multipotent progenitors to Ig-secreting cells." in: Blood, Vol. 92, Issue 12, pp. 4509-20, (1999) (PubMed).

    Janowska-Wieczorek, Marquez, Nabholtz, Cabuhat, Montaño, Chang, Rozmus, Russell, Edwards, Turner: "Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane." in: Blood, Vol. 93, Issue 10, pp. 3379-90, (1999) (PubMed).

  • Target
    CD34
    Alternative Name
    CD34 (CD34 Products)
    Synonyms
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    Background
    CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
    Gene ID
    947
    UniProt
    P28906
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