Polybromo 1 antibody (AA 1-306)

Catalog No. ABIN1111129

Product Details anti-Polybromo 1 Antibody

Target: Polybromo 1 (PBRM1)

Binding Specificity: AA 1-306

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Polybromo 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Polybromo 1 (PBRM1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PBRM1. It has been selected for its ability to recognize PBRM1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Polybromo 1 (PBRM1) corresdonding to Met1~Ala306 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Polybromo 1

Target: Polybromo 1 (PBRM1)

Alternative Name: Polybromo 1 (PBRM1 Products)

Synonyms: pb1 antibody, BAF180 antibody, PB1 antibody, 2610016F04Rik antibody, AI507524 antibody, Pb1 antibody, RGD1565549 antibody, polybromo 1 antibody, polybromo 1 L homeolog antibody, protein polybromo-1 antibody, PBRM1 antibody, pbrm1.L antibody, LOC100170602 antibody, pbrm1 antibody, Pbrm1 antibody

Background: PB1, BAF180, BRG1-associated factor 180

Molecular Weight: 62.1 kDa