Integrin Alpha2b antibody (PerCP)

Catalog No. ABIN1112136

Product Details anti-Integrin Alpha2b Antibody (PerCP)

Target: Integrin Alpha2b (CD41)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin Alpha2b antibody is conjugated to PerCP

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD41 PerCP is recommended for use in flow cytometry for identification of Erythroblast.

Clone: HIP8

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Peridin-chlorophyll-protein complex (Prozyme).

Sample Collection: 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD41 PerCP and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0, 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing Antibody Stabilizer.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 24-02-2010 HT-0041-PP-1 CD41 PerCP-> 10 10 10 10 100 1 2 3 4 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

References for anti-Integrin Alpha2b antibody (PerCP) (ABIN1112136)

Menéndez, del Cañizo, Orfao: "Immunophenotypic characteristics of PB-mobilised CD34+ hematopoietic progenitor cells." in: Journal of biological regulators and homeostatic agents, Vol. 15, Issue 1, pp. 53-61, (2001) (PubMed).

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: CD41 (CD41 Products)

Synonyms: AI172977 antibody, CD41 antibody, CD41B antibody, GpIIb antibody, alphaIIb antibody, BDPLT16 antibody, BDPLT2 antibody, GP2B antibody, GPIIb antibody, GT antibody, GTA antibody, HPA3 antibody, integrin alpha 2b antibody, integrin subunit alpha 2b antibody, Itga2b antibody, ITGA2B antibody

Background: This clone has been clustered to CD41 in the fourth international Workshop on Human White Cell differentiation Antigens. The antibody recognises the intact platelet gpIIb/IIIa complex, it does not bind to dissociated gpIIb or gpIIIa only to gpIIb in the intact complex. This is found on platelets, megakaryocytes and monocytes. Acute megakaryoblastic l leukaemia cells are positive for the gpIIb/IIIa antigen whereas common myeloid leukaemias are negative. CD41 is expressed on platelets and megakaryocytes. CD41 is the integrin aIIb subunit that is expressed as a heterodimer non-covalently associated with the integrin b3 subunit CD61. The association between CD41 and CD61 is calcium dependent.

Pathways: Integrin Complex