CD52 antibody (Biotin)

Catalog No. ABIN1112182

Product Details anti-CD52 Antibody (Biotin)

Target: CD52

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD52 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD52 Biotin is recommended for use in flow cytometry.

Clone: HI186

Isotype: IgG2b

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 µl, 1/100, of the suggested working dilution to label 10^6 cells.

Comment:

BIOTIN (Sigma) Anti-CD52, HI185, was included in the Sixth International Workshops on Human Leucocyte Defferentiation Antigens.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing Antibody Stabilizer.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD52

Target: CD52

Alternative Name: CD52 (CD52 Products)

Background: HI186 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.