KLRK1 antibody (FITC)

Catalog No. ABIN1112444

Product Details anti-KLRK1 Antibody (FITC)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This KLRK1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Anti-Mouse CD314 PE is recommended for use in flow cytometry for identification of CD314 also known as NKG2D. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.

Immunogen: Soluble mouse NKG2D Species

Clone: C7

Isotype: IgG

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 0,25 µg/10^6 cells

Comment:

R-Phycoerythrin (R-PE). Abs/Em: 545/575nm.

Reagent Preparation:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD314 PE and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: CD314 (KLRK1 Products)

Synonyms: KLRK1 antibody, NKG2D antibody, CD314 antibody, D12S2489E antibody, KLR antibody, NKG2-D antibody, D6H12S2489E antibody, Nkg2d antibody, Nkrp2 antibody, killer cell lectin like receptor K1 antibody, killer cell lectin-like receptor subfamily K, member 1 antibody, NKG2D protein antibody, KLRK1 antibody, Klrk1 antibody, NKG2D antibody

Background: NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans, present in both mice and humans. NKG2D ligands trigger cytokine (IFN-gama, GM-CSF, TNF-alpha, MIP1beta and others) and granzyme release from NK cells.

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process