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F4/80 antibody

EMR1 Reactivity: Mouse FACS, WB, IHC (p), IP, IHC (fro), RIA, Func Host: Rat Monoclonal Cl:A3-1 unconjugated
Catalog No. ABIN111856
  • Target See all F4/80 (EMR1) Antibodies
    F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
    Reactivity
    • 92
    • 52
    • 30
    • 4
    • 4
    • 1
    Mouse
    Host
    • 55
    • 47
    • 4
    • 3
    • 2
    • 1
    Rat
    Clonality
    • 55
    • 49
    • 2
    Monoclonal
    Conjugate
    • 43
    • 10
    • 9
    • 8
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This F4/80 antibody is un-conjugated
    Application
    • 80
    • 56
    • 25
    • 17
    • 13
    • 13
    • 12
    • 11
    • 11
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Functional Studies (Func)
    Specificity
    This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
    Purification
    Affinity Chromatography on Protein G
    Endotoxin Level
    Low Endotoxin
    Immunogen
    Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
    Clone
    Cl:A3-1
    Isotype
    IgG2b
    Top Product
    Discover our top product EMR1 Primary Antibody
  • Application Notes
    Western Blot. Immunoprecipitation. Radioimmunoassays. Functional Assays. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Resin Sections. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires pre-treatment prior to staining.
    Protocol
    1. Enzyme pre-treatment using Proteinase K (recommended for tissues fixed for 24 hoursin neutral buffered formalin, NBF): ReagentsA. TE buffer (50 mM Tris base, 1 mM EDTA, pH 8. 0)Tris Base, 6. 10 gEDTA, 0. 37 gDistilled water, 1000 ml
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4, None, None
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Target
    F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
    Alternative Name
    Macrophage F4/80 Antigen (EMR1 Products)
    Synonyms
    EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody
    Background
    F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
    Gene ID
    13733
    NCBI Accession
    NP_034260
    UniProt
    Q61549
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