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S100A8/A9 Complex (Calprotectin) antibody

S100A8/A9 Reactivity: Human IHC (p), EIA, IHC (fro) Host: Mouse Monoclonal 2-70E-011 unconjugated
Catalog No. ABIN111882
  • Target See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) Antibodies
    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
    Reactivity
    • 32
    • 13
    • 13
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 9
    • 9
    • 9
    Human
    Host
    • 15
    • 14
    • 3
    Mouse
    Clonality
    • 18
    • 14
    Monoclonal
    Conjugate
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated
    Application
    • 15
    • 13
    • 12
    • 7
    • 7
    • 4
    • 3
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody is ideally suited for the detection of early inflammatory macrophages and thus for the classification of acute stage inflammation in tissue sections and in smears, the characterization of tumorous tissues and the in vitro monitoring of peripheral blood cell cultures. Clone 27E10 is unique in that it recognizes an epitope on the MRP8/14 heterocomplex that is not exposed on the individual subunits MRP8 or MRP14. The antibody reacts with Human subpopulations of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Antigen Distribution Isolated Cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24-48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue Sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).Antigen Distribution
    Cross-Reactivity (Details)
    Species reactivity (tested):Human. The antibody also stains a subpopulation of macrophages of Rhesus Monkey and Bovine tissues. It does not react with Swine tissues.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Cultured Human monocytes. Remarks: The antigen is MRP8/14 (Calprotectin), the epitope involves parts of both subunits MRP8 and MRP14.
    Clone
    2-70E-011
    Isotype
    IgG1
    Top Product
    Discover our top product S100A8/A9 Primary Antibody
  • Application Notes
    ELISAImmunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200), Proteinase K pre-treatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Dot blots.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalum
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water (= 0.2 mg/mL Stock Solution).
    Concentration
    0.2 mg/mL
    Buffer
    Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!.
  • Ghavami, Rashedi, Dattilo, Eshraghi, Chazin, Hashemi, Wesselborg, Kerkhoff, Los: "S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1484-92, (2008) (PubMed).

    Altwegg, Neidhart, Hersberger, Müller, Eberli, Corti, Roffi, Sütsch, Gay, von Eckardstein, Wischnewsky, Lüscher, Maier: "Myeloid-related protein 8/14 complex is released by monocytes and granulocytes at the site of coronary occlusion: a novel, early, and sensitive marker of acute coronary syndromes." in: European heart journal, Vol. 28, Issue 8, pp. 941-8, (2007) (PubMed).

  • Target
    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
    Alternative Name
    MRP8 / MRP14 (S100A8/A9 Products)
    Synonyms
    60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, S100 calcium binding protein A9 antibody, S100A9 antibody
    Background
    MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. The antigen is produced by the heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.Synonyms: CAGA, CAGB, CFAG, Calgranulin A/B, Calprotectin, L1 Protein, MRP-14, MRP-8, P14, P8
    Pathways
    S100 Proteins
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