Streptococcus Group A antibody (FITC)

Catalog No. ABIN112830

Product Details anti-Streptococcus Group A Antibody (FITC)

Target: Streptococcus Group A

Reactivity: Streptococcus

Host: Goat

Clonality: Polyclonal

Conjugate: This Streptococcus Group A antibody is conjugated to FITC

Application: Immunofluorescence (IF)

Characteristics: Molar Ratio: F:P ratio = 5.4

Purification: Immunoaffinity chromatography

Immunogen: Streptococci, Group A

Application Details

Application Notes: ELISA (functions both as capture and detection antibody). Immunofluorescence assay: a starting range of 1: 10-1: 50 is suggested. Direct Fluorescence assay staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Pre-block slidewith 10 % normal goat serum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide as preservative and 10 mg/mL BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Streptococcus Group A

Target: Streptococcus Group A

Abstract: Streptococcus Group A Products

Target Type: Bacteria

Background: The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nose and pharynx. Streptococci can be divided into many groups on the basis of antigenic differences in group-specific polysaccharides located in the bacterial cell wall. More than 20 serologic groups have been identified and designated by letters, eg, A, B, C. Group A streptococci, cause a wide-range of disease in humans, from mild sore throats to life-threatening invasive disease such as necrotising fasciitis. Streptococcus pyogenes (a group A Streptococcus) is one of the most important pathogens encountered in clinical practice. Group A streptococci (GAS) are gram-positive, nonmotile, non-spore-forming organisms that appear as pairs or short-to-moderate-sized chains. Group A organisms can be identified by enzyme immunoassays.