The LDL receptor (160 kDa mature or glycosylated receptor, 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis. The antibody to LDL-R reacts specifically with Human LDL-R and is suitable for use in Flow Cytometry and Immunoblotting. First data show that the antibody does not inhibit binding of LDL. In Immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Immunoaffinity chromatography
Immunogen
Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5).
LDLR
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow cytometry: 1: 10. Receptor binding studies. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS with Penicillin / Streptomycin for antibiotic protection
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Akazawa, Date, Morikawa, Murayama, Miyamoto, Kaga, Barth, Baumert, Dubuisson, Wakita: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." in: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).
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Background
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.Synonyms: LDL receptor, LDLR, Low-density lipoprotein receptor