H2-Q7 antibody (Histocompatibility 2, Q Region Locus 7)

Catalog No. ABIN114301

Product Details anti-H2-Q7 Antibody

Target:

H2-Q7

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2-Q7 antibody is un-conjugated

Application: Cytotoxicity Test (CyTox)

Specificity: This antibody reacts to MHC Class I Qa.m7. This monoclonal Qa.m7 antibody detects the Qa.m7 antigenic determinant on molecule coded by H-2 linked by mapping within the Qa region. It is possible that this specificity represents a discrete epitope on previously described Loci (Qa-2) rather than a product of new Qa loci, which may code for antigenically distinct molecules. In fact, this Qa.m7 antibody defines a Qa antigen which appears to be molecularly similar or identical to antigen defined by antibodies to other Qa specification (i.e. Qa.m2, Qa.m8, Qa.m9). Biochemical studies have inidcated that Qa.m7 is found on a molecule consisting of a 39,000d heavy chain and a 12,000d light chain wgich is B2, Microglobulin. Qa.m7 is distinguished from other Qa specificities on the basis of unique strain and distribution pattern obtained. It has been identified on >90 % of spleen and lymph node cells and ~67 % of bone marrow cells but is absent on lymphocytes. This antibody, in conjunction with others (i.e. anti-Qa.m8 antibody) could prove useful in defining subsets of functional cells. Examples: Qa.m7: >90 % T cells, 70 % B cells. Qa.m8: 70 % T cells, 0 % B cells. As each of these antigens are present on subsets of T cells (Qa.m8), B cells (Qa.m7) and bone marrow cells (Qa.m7 and Qa.m8), it may be used to define subsets of functional cells.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Ascites

Immunogen: Recipient: BALB/c By Donor: B6.Ly1a Fusion Partner: P3-NSI/1-Ag4(NS-1)

Clone: 5035-50-1

Isotype: IgM

Application Details

Application Notes: Cytotoxicity Assay. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF Qa. m7 POSITIVELYMPHOCYTES1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-M density cell separation medium. After washing adjust thecell concentration to 1 x 10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration 1/20 and mix. Alternately, pellet the cells andresuspend in antibody diluted 1/20 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Low-Tox®-M Rabbit Complement3, diluted to theappropriate concentration in Cytotoxicity Medium. (Recommended concentration includedwith each batch of Low-Tox®-M Rabbit Complement. )6. Incubate for 60 minutes at 37°C. 7. Monitor for percent cytotoxicity at this stage, before further processing. For this purpose,remove a small sample from each tube, dilute 1/10 with medium, and add 1/10 volume of

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 0.5 mL distilled water.

Precaution of Use: Do Not Use Sodium Azide as preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below.

Target Details for H2-Q7

Target:

H2-Q7

Alternative Name: QA-2 Antigen (H2-Q7 Antibody Abstract)

Synonyms: H-2Q7, Ped, Q9, Qa-2, Qa-7, Qa7, histocompatibility 2, Q region locus 7, H2-Q7

Background: The Qa region, so named because of the closely linked Qa 1-9 loci, is situated on murine chromosome 17 between the H-2D and T1a loci. These Qa loci code for cell surface alloantigens expressed on some lymphoid and myeloid cells. Their expression appears to be controlled by a gene or genes closely linked to the H-2D locus and they have been identified as being structurally related to the murine H-2 antigens. Although no functional role for Qa antigens has been determined, the restricted and differential distribution of Qa determinants on hemopoietic and lymphoid cells raises the possibility that they may not be simply differentiation markers but may play a role in hematopoetic differentiation.Synonyms: H-2 class I histocompatibility antigen, H2-Q7, Q7 alpha chain

Gene ID: 100044019

NCBI Accession: NP_034524

UniProt: P14429