Macrophages antibody

Catalog No. ABIN114398

The Rabbit Polyclonal anti-Macrophages antibody is suitable to detect Macrophages in samples from Mouse. It has been validated for IHC (fro), IHC (p) and Func.

Quick Overview for Macrophages antibody (ABIN114398)

Target: Macrophages

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Product Details

Purification: Affinity Purified

Application Details

Application Notes: Cytotoxic Assays. Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Test Type: For Cytotoxic Antibodies: Modified Colorimetric Microtiter Assay (Ref. 1)Results: Antisera of this lot diluted 1/20 exhibits greater than 75% cytotoxicity on MouseMacrophages. Antisera of this lot diluted 1/20 exhibits less than 10% cytotoxicity on Mouse thymocytes orsplenocytes. For Agglutinating Antibodies: Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1hr. Agglutinationdetermined by microscopic observations. Results: Antisera of this lot strongly agglutinates mouse macrophages at a dilution of 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Macrophages

Background: Synonyms: Macrophage marker