Rabbit anti-Human IgG Antibody

Details for Product No. ABIN115322, Supplier: Log in to see
Antigen
Reactivity
Human
2913
2617
2172
1556
1393
751
601
456
426
389
365
357
295
280
265
145
132
113
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Host
Rabbit
6502
4929
1660
1009
521
357
93
67
54
19
8
5
5
5
3
1
1
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Supplier
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Supplier Product No.
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Immunogen IgG isolated from a pool of normal Human sera.
Purification Purified
Background IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75 % of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66 %), IgG2 (23 %), IgG3 (7 %) and IgG4 (4 %).Synonyms: Human Immunoglobulin G
Application Notes Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffinembedded tissue sections. Recommended Positive Control: Tonsil

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 18.9 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for Rabbit anti-Human IgG antibody (ABIN115322) Formalin fixed paraffin embedded human tonsil stained with IgG antibody. ABIN115322
Background publications Koslov, Maupin, Pradhan, Morrow, Rimm: "Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 27301-6, 1997 (PubMed).