Apolipoprotein C-II antibody

Catalog No. ABIN116545

This Goat Polyclonal antibody specifically detects Apolipoprotein C-II in WB, IP, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for Apolipoprotein C-II antibody (ABIN116545)

Target: Apolipoprotein C-II (APOC2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Apolipoprotein C-II antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-Apolipoprotein C-II Antibody

Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-II and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Purification: Immunoaffinity chromatography

Immunogen: ApoLipoprotein Type C-II produced synthetically in full-length form (not selected epitopes) using conventional peptide technology

Application Details

Application Notes: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitationof antigen in serum using a standard curve, for Immunoprecipitation and for Westernblotting for highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by Immunoblot and found to be

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 0.01 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Apolipoprotein C-II

Target: Apolipoprotein C-II (APOC2)

Alternative Name: Apolipoprotein C II (Apo CII)

Background: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-II (ap °CII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). Ap °CII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.Synonyms: APC2, Apo-CII, Ap °C-II, Ap °C2, Apolipoprotein C-II, Apolipoprotein C2

Gene ID: 344

NCBI Accession: NP_000474

UniProt: P02655

Pathways: Lipid Metabolism