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Collagen IV antibody

COL4 Reactivity: Human, Mammalian WB, IHC (p), IHC (fro), IP, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN116560
  • Target See all Collagen IV (COL4) Antibodies
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Reactivity
    • 92
    • 44
    • 42
    • 23
    • 16
    • 11
    • 5
    • 2
    Human, Mammalian
    Host
    • 60
    • 28
    • 13
    Rabbit
    Clonality
    • 72
    • 29
    Polyclonal
    Conjugate
    • 51
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Collagen IV antibody is un-conjugated
    Application
    • 63
    • 51
    • 31
    • 29
    • 28
    • 26
    • 26
    • 26
    • 20
    • 10
    • 9
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Purification
    Immunoaffinity Chromatography.
    Immunogen
    Collagen type IV purified from Human and Bovine placenta.
    Top Product
    Discover our top product COL4 Primary Antibody
  • Application Notes
    Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for native(non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitivequalitative analysis. Recommended Dilutions: ELISA 1/5,000-1/20,000. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200 (prepare tissues as frozen tissue sections). Note: This product was assayed by Immunoblot and found to be reactive against CollagenIV at a dilution of 1/5,000-1/10,000. This product was also assayed against 1.0 μg of Collagen IV in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Rodriguez-Teja, Gronau, Breit, Zhang, Minamidate, Caley, McCarthy, Cox, Erler, Gaughan, Darby, Robson, Mauri, Waxman, Sturge: "AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival." in: The Journal of pathology, Vol. 235, Issue 4, pp. 581-92, (2015) (PubMed).

    Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

    Sasse, Malan, Fleischmann, Roell, Gustafsson, Bostani, Fan, Kolbe, Breitbach, Addicks, Welz, Brem, Hescheler, Aszodi, Costell, Bloch, Fleischmann: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).

  • Target
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Alternative Name
    Collagen Type IV (COL4 Products)
    Synonyms
    collagen, type IV antibody, Let-2 antibody
    Background
    Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens were extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: COL4A1
    Gene ID
    9606
    UniProt
    P02462
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