IL1RN antibody

Catalog No. ABIN116864

Product Details anti-IL1RN Antibody

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Purification: DEAE Chromatography.

Immunogen: Prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.

Application Details

Application Notes: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for usein Neutralizations, ELISA, Radioimmunoassays, Western blotting, Immunohistochemistryand Flow cytometry. Reactivity in other immunoassays is unknown. Note: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L]and TMB as a substrate. A working dilution range of 1: 400 to 1: 800 is suggested for thisapplication. This product has been assayed by ELISA against IL-1Receptor Antagonist usingHRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes atroom temperature. A working dilution range of 1: 500 to 1: 1,000 is suggested for thisproduct. For use in ELISA formats, this antibody is best used as the second antibody incombination with a monoclonal antibody as a capture antibody. This product has beenassayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry. This product has been assayed by radioimmunoassay againstantigen. A dilution of 1: 8,000 is suggested for this immunoassay. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN / IL1RA (IL1RN Products)

Synonyms: DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody

Background: IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein

Gene ID: 9606

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints