TNFRSF10A antibody

Catalog No. ABIN1169237

Product Details anti-TNFRSF10A Antibody

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF10A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Specificity: Recognizes human TRAIL-R1.

Cross-Reactivity: Human

Cross-Reactivity (Details): Does not cross-react with human TRAIL-R2, -R3 or -R4.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human TRAIL-R1 (DR4).

Clone: HS101

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-TNFRSF10A antibody (ABIN1169237)

Secchiero, Melloni, Heikinheimo, Mannisto, Di Pietro, Iacone, Zauli: "TRAIL regulates normal erythroid maturation through an ERK-dependent pathway." in: Blood, Vol. 103, Issue 2, pp. 517-22, (2004) (PubMed).

Tecchio, Huber, Scapini, Calzetti, Margotto, Todeschini, Pilla, Martinelli, Pizzolo, Rivoltini, Cassatella: "IFNalpha-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells." in: Blood, Vol. 103, Issue 10, pp. 3837-44, (2004) (PubMed).

Ganten, Haas, Sykora, Stahl, Sprick, Fas, Krueger, Weigand, Grosse-Wilde, Stremmel, Krammer, Walczak: "Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs." in: Cell death and differentiation, Vol. 11 Suppl 1, pp. S86-96, (2004) (PubMed).

Leverkus, Sprick, Wachter, Mengling, Baumann, Serfling, Bröcker, Goebeler, Neumann, Walczak: "Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, (2003) (PubMed).

Washburn, Weigand, Grosse-Wilde, Janke, Stahl, Rieser, Sprick, Schirrmacher, Walczak: "TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1814-21, (2003) (PubMed).

Sprick, Rieser, Stahl, Grosse-Wilde, Weigand, Walczak: "Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8." in: The EMBO journal, Vol. 21, Issue 17, pp. 4520-30, (2002) (PubMed).

Target Details for TNFRSF10A

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL-R1 (TNFRSF10A Products)

Synonyms: APO2 antibody, CD261 antibody, DR4 antibody, TRAILR-1 antibody, TRAILR1 antibody, TNFRSF10A antibody, TNF receptor superfamily member 10a antibody, tumor necrosis factor receptor superfamily member 26 antibody, tumor necrosis factor receptor superfamily, member 10a antibody, TNFRSF10A antibody, LOC510798 antibody

Background: TRAIL-R1 is a receptor for the cytotoxic ligand TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappaB.

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity