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Avidin antibody (HRP)

AVD Reactivity: Chicken WB, IHC (fro), IHC (p), EIA Host: Rabbit Polyclonal HRP
Catalog No. ABIN116963
  • Target See all Avidin (AVD) Antibodies
    Avidin (AVD)
    Reactivity
    • 8
    • 7
    • 4
    Chicken
    Host
    • 10
    • 7
    • 1
    Rabbit
    Clonality
    • 11
    • 7
    Polyclonal
    Conjugate
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This Avidin antibody is conjugated to HRP
    Application
    • 13
    • 13
    • 10
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown.
    Purification
    Immunoaffinity chromatography.
    Immunogen
    Avidin [Hen Egg White].
    Top Product
    Discover our top product AVD Primary Antibody
  • Application Notes
    Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: This product has been assayed against 1.0 μg of Avidin [Hen EggWhite ] in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,000 to 1: 27,000 of the reconstitutionconcentration is suggested for this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 2.0 mL of deionized water (or equivalent).
    Concentration
    10.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • Target
    Avidin (AVD)
    Alternative Name
    Avidin (AVD Products)
    Synonyms
    avidin antibody, avd antibody, LOC723980 antibody, AVD antibody
    Background
    The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
    Gene ID
    396260
    NCBI Accession
    NP_990651
    UniProt
    P02701
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