Rabbit anti-Human IgG (Fc Region) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN117268

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunomicroscopy (IM)

Characteristics: Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG F(c) fragment.

Application Details

Application Notes: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Note: This product has been assayed against 1.0 μg of Human IgG in a standard captureELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1/10,000-1/100,000 of the reconstitution concentration is suggestedfor this product. Recommended Dilutions: ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabilizer.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin G