Goat anti-Human IgA, IgG, IgM (Whole Molecule) Antibody (HRP) - Preadsorbed

Catalog No. ABIN117312

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Whole Molecule

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG, IgA and IgM whole molecules

Application Details

Application Notes: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended dilutionsELISA 1/10,000-1/70,000. Western blot 1/1,000-1/5,000. Immunohistochemistry 1/500-1/2,500.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as a preservative.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References

Smart, Goecke, Ramm, Petersen, Lenz, Haverich, Niemann, Hilfiker: "Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves." in: Xenotransplantation, Vol. 28, Issue 1, pp. e12646, (2021) (PubMed).

Ramm, Hartmann, Tudorache, Haverich, Hilfiker: "No evidence for αGal epitope transfer from media containing FCS onto human endothelial cells in culture." in: Xenotransplantation, Vol. 22, Issue 5, pp. 345-55, (2015) (PubMed).

von Rhein, Hunfeld, Ludwig: "Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection." in: Infection and immunity, Vol. 74, Issue 11, pp. 6505-8, (2006) (PubMed).

Target Details

Target: IgA, IgG, IgM

Abstract: IgA, IgG, IgM Products

Target Type: Antibody

Background: Synonyms: Human IgA, Human IgG, Human IgM