Vinculin antibody (AA 1020-1134)

Catalog No. ABIN1175095

Product Details anti-Vinculin Antibody

Target: Vinculin (VCL)

Binding Specificity: AA 1020-1134

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Vinculin (VCL)

Specificity: The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Vinculin (VCL) corresdonding to Lys1020~Gln1134 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL

Immunohistochemistry: 5-50 μg/mL

Immunocytochemistry: 5-50 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin (VCL Products)

Synonyms: CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody

Background: MVCL, Metavinculin

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2