SIRT4 antibody (AA 60-255)

Catalog No. ABIN1176392

Product Details anti-SIRT4 Antibody

Target: SIRT4 (Sirtuin 4 (SIRT4))

Binding Specificity: AA 60-255

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Sirtuin 4 (SIRT4)

Specificity: The antibody is a rabbit polyclonal antibody raised against SIRT4. It has been selected for its ability to recognize SIRT4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Sirtuin 4 (SIRT4) corresdonding to Ala60~Val255 (Accession # G3V641)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: Sirtuin 4 (SIRT4 Products)

Synonyms: SIRT4 antibody, im:7139453 antibody, SIR2L4 antibody, 4930596O17Rik antibody, sirtuin 4 antibody, SIRT4 antibody, sirt4 antibody, Sirt4 antibody

Background: SIR2L4, Silent Mating Type Information Regulation 2 Homolog 4, SIR2-like protein 4, NAD-dependent protein deacetylase sirtuin-4

Pathways: Negative Regulation of Hormone Secretion