This Alexa Fluor® 647-conjugated antibody is suitable for intracellular staining of cell lines and primary cells using BD Cytofix™ Fixation Buffer or BD™ Phosflow Lyse/Fix Buffer. Although this antibody can be used with BD™ Phosflow Perm/Wash Buffer I or BD™ Phosflow Perm Buffers II, III or IV, it performs optimally when used with BD™ Phosflow Perm Buffer II.
Sample Volume
5 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, (2000) (PubMed).
Target
NFKBIA
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
IKBA antibody, MAD-3 antibody, NFKBI antibody, AI462015 antibody, Nfkbi antibody, RL/IF-1 antibody, ECI-6/IKBA antibody, NFKBIA antibody, ikbaB antibody, wu:fb81f08 antibody, zgc:56666 antibody, zgc:77616 antibody, ikbaA antibody, wu:fj16a07 antibody, zgc:85769 antibody, NFKB inhibitor alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antibody, NFKBIA antibody, Nfkbia antibody, nfkbiab antibody, nfkbiaa antibody
Background
NF-kappaB is a transcription factor that is a member of the mammalian NF-kappaB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. In resting cells, IkappaBalpha binds to and maintains NF-kappaB in the cytoplasm by blocking the nuclear localization sequences of NF-kappaB. In the cellular response to an extracellular signal, IkappaBalpha is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-kappaB to translocate to the nucleus. Once in the nucleus, NF-kappaB can induce the transcription of IkappaBalpha thereby renewing the cycle so that IkappaBalpha can form a complex with NF-kappaB and maintain it in its cytoplasmic location. IkappaBalpha -/- mice show an increased level of NF-kappaB activity and have been shown to die soon after birth. The 25/IkBa/MAD-3 monoclonal antibody recognizes human IkappaBalpha regardless of phosphorylation status and does not cross-react with mouse IkappaBalpha. Synonyms: MAD-3, I-kappa-B-alpha, IkB-alpha, NFKBIA, NF-kappa-B inhibitor alpha