CD45 antibody

Catalog No. ABIN1176960

Product Details anti-CD45 Antibody

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells

Clone: RA3-6B2

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD45 antibody (ABIN1176960)

Bleesing, Morrow, Uzel, Fleisher: "Human T cell activation induces the expression of a novel CD45 isoform that is analogous to murine B220 and is associated with altered O-glycan synthesis and onset of apoptosis." in: Cellular immunology, Vol. 213, Issue 1, pp. 72-81, (2001) (PubMed).

Driver, McHeyzer-Williams, Cool, Stetson, McHeyzer-Williams: "Development and maintenance of a B220- memory B cell compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1393-405, (2001) (PubMed).

Kobata, Takasaki, Asahara, Hong, Masuko-Hongo, Kato, Hirose, Shirai, Kayagaki, Yagita, Okumura, Nishioka: "Apoptosis with FasL+ cell infiltration in the periphery and thymus of corrected autoimmune mice." in: Immunology, Vol. 92, Issue 2, pp. 206-13, (1998) (PubMed).

Sagara, Sugaya, Tokoro, Tanaka, Takano, Kodama, Nakauchi, Takahama: "B220 expression by T lymphoid progenitor cells in mouse fetal liver." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 666-76, (1997) (PubMed).

Puzanov, Bennett, Kumar: "IL-15 can substitute for the marrow microenvironment in the differentiation of natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4282-5, (1996) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Renno, Hahne, Tschopp, MacDonald: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, (1996) (PubMed).

Rolink, ten Boekel, Melchers, Fearon, Krop, Andersson: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, (1996) (PubMed).

Domiati-Saad, Ogle, Justement: "Administration of anti-CD45 mAb specific for a B cell-restricted epitope abrogates the B cell response to a T-dependent antigen in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5936-47, (1994) (PubMed).

George, Rath, Shroff, Wang, Durdik: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).

Laouar, Ezine: "In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3948-55, (1994) (PubMed).

Ballas, Rasmussen: "Lymphokine-activated killer cells. VII. IL-4 induces an NK1.1+CD8 alpha+beta- TCR-alpha beta B220+ lymphokine-activated killer subset." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 1, pp. 17-30, (1993) (PubMed).

Allman, Ferguson, Cancro: "Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2533-40, (1992) (PubMed).

Hathcock, Hirano, Murakami, Hodes: "CD45 expression by B cells. Expression of different CD45 isoforms by subpopulations of activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2286-94, (1992) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Asensi, Kimeno, Kawamura, Sakumoto, Nomoto: "Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies." in: Immunology, Vol. 68, Issue 2, pp. 204-8, (1989) (PubMed).

Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, (1983) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45R/B220 (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker, however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.
Synonyms: B220, Ly-5, CD45R, LCA, Ptprc, Protein tyrosine phosphatase receptor type C

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling