CD103 antibody

Catalog No. ABIN1176991

Product Details anti-CD103 Antibody

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD103 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Intestinal Epithelial Cells

Clone: M290

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD103 antibody (ABIN1176991)

Feng, Wang, Yuan, Parker, Farber, Hadley: "CD103 expression is required for destruction of pancreatic islet allografts by CD8(+) T cells." in: The Journal of experimental medicine, Vol. 196, Issue 7, pp. 877-86, (2002) (PubMed).

Hadley, Bartlett, Via, Rostapshova, Moainie: "The epithelial cell-specific integrin, CD103 (alpha E integrin), defines a novel subset of alloreactive CD8+ CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 3748-56, (1997) (PubMed).

Karecla, Green, Bowden, Coadwell, Kilshaw: "Identification of a binding site for integrin alphaEbeta7 in the N-terminal domain of E-cadherin." in: The Journal of biological chemistry, Vol. 271, Issue 48, pp. 30909-15, (1997) (PubMed).

Andrew, Rott, Kilshaw, Butcher: "Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes." in: European journal of immunology, Vol. 26, Issue 4, pp. 897-905, (1996) (PubMed).

Karecla, Bowden, Green, Kilshaw: "Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7)." in: European journal of immunology, Vol. 25, Issue 3, pp. 852-6, (1995) (PubMed).

Lefrançois, Barrett, Havran, Puddington: "Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells." in: European journal of immunology, Vol. 24, Issue 3, pp. 635-40, (1994) (PubMed).

Roberts, Kilshaw: "The mucosal T cell integrin alpha M290 beta 7 recognizes a ligand on mucosal epithelial cell lines." in: European journal of immunology, Vol. 23, Issue 7, pp. 1630-5, (1993) (PubMed).

Kilshaw, Murant: "Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system." in: European journal of immunology, Vol. 21, Issue 10, pp. 2591-7, (1991) (PubMed).

Kilshaw, Murant: "A new surface antigen on intraepithelial lymphocytes in the intestine." in: European journal of immunology, Vol. 20, Issue 10, pp. 2201-7, (1990) (PubMed).

Kilshaw, Baker: "A unique surface antigen on intraepithelial lymphocytes in the mouse." in: Immunology letters, Vol. 18, Issue 2, pp. 149-54, (1988) (PubMed).

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: CD103 (ITGAE Products)

Synonyms: A530055J10 antibody, CD103 antibody, aM290 antibody, alpha-E1 antibody, alpha-M290 antibody, HUMINAE antibody, integrin alpha E, epithelial-associated antibody, integrin subunit alpha E antibody, Itgae antibody, ITGAE antibody

Background: The M290 antibody reacts with the alpha chain of alphaIELbeta7 integrin. CD103 has a unique and fairly restricted tissue distribution. It is expressed on almost all intestinal intraepithelial lymphocytes (IEL), on dendritic epidermal T cells (DEC), on subpopulations of peripheral T cells, and on distinct subsets of fetal, neonatal, and adult thymocytes. E-cadherin is the epithelial-cell ligand for alphaIELbeta7 integrin. The ordered expression of alphaIEL during thymocyte development (which occurs under the influence of the thymic epithelium), the high level of expression of alphaIEL on those peripheral T cells found in epithelial tissues (IEL and DEC), and the expression of CD103 on a subset of CD8+ lymphocytes responding to allogeneic epithelial cells suggest that alphaIELbeta7 integrin may have a common role in the interactions of T lymphocytes with epithelia during T-cell maturation and effector functions. CD103 is thought to play a role in allograft rejection. The M290 antibody is reported to efficiently inhibit alphaIELbeta7-mediated adhesion in in vitro assays.
Synonyms: Integrin alphaIEL chain