CD14 antibody

Catalog No. ABIN1176993

Product Details anti-CD14 Antibody

Target: CD14

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse monocyte/macrophage cell line

Clone: 4C1-CD14

Isotype: IgG2b kappa

Application Details

Application Notes: For western blot and flow cytometric analysis, investigators may find Rat Anti-Mouse CD14 mAb rmC5-3 (Cat. No. 553738), FITC Rat Anti-Mouse CD14 (Cat. No. 553739) and/or PE Rat Anti-Mouse CD14 (Cat. No. 553740) to be useful.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD14 antibody (ABIN1176993)

Tasaka, Ishizaka, Yamada, Shimizu, Koh, Hasegawa, Adachi, Yamaguchi: "Effect of CD14 blockade on endotoxin-induced acute lung injury in mice." in: American journal of respiratory cell and molecular biology, Vol. 29, Issue 2, pp. 252-8, (2003) (PubMed).

Akashi, Saitoh, Wakabayashi, Kikuchi, Takamura, Nagai, Kusumoto, Fukase, Kusumoto, Adachi, Kosugi, Miyake: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).

Triantafilou, Triantafilou: "Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster." in: Trends in immunology, Vol. 23, Issue 6, pp. 301-4, (2002) (PubMed).

Nagaoka, Hirota, Niyonsaba, Hirata, Adachi, Tamura, Heumann: "Cathelicidin family of antibacterial peptides CAP18 and CAP11 inhibit the expression of TNF-alpha by blocking the binding of LPS to CD14(+) cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3329-38, (2001) (PubMed).

Yang, Sugawara, Monodane, Nishijima, Adachi, Akashi, Miyake, Hase, Takada: "Micrococcus luteus teichuronic acids activate human and murine monocytic cells in a CD14- and toll-like receptor 4-dependent manner." in: Infection and immunity, Vol. 69, Issue 4, pp. 2025-30, (2001) (PubMed).

Le Roy, Di Padova, Adachi, Glauser, Calandra, Heumann: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, (2001) (PubMed).

Ziegler-Heitbrock: "Heterogeneity of human blood monocytes: the CD14+ CD16+ subpopulation." in: Immunology today, Vol. 17, Issue 9, pp. 424-8, (1997) (PubMed).

Haziot, Ferrero, Köntgen, Hijiya, Yamamoto, Silver, Stewart, Goyert: "Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice." in: Immunity, Vol. 4, Issue 4, pp. 407-14, (1996) (PubMed).

Matsuura, Ishida, Setoguchi, Higuchi, Akizuki, Yamamoto: "Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide." in: The Journal of experimental medicine, Vol. 179, Issue 5, pp. 1671-6, (1994) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: The 4C1/CD14 antibody reacts with CD14, a ~55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein. Flow cytometric analysis demonstrates that 4C1/CD14 antibody stains CD14 transfectants, the monocyte/macrophage cell line RAW 264.7 peritoneal exudate neutrophils and macrophages, and cultured bone marrow-derived macrophages, but not undifferentiated bone marrow cells, splenocytes, or peripheral blood leukocytes. This staining pattern is similar to that of the alternate anti-mouse CD14 mAb rmC5-3, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils. Therefore, data suggest that CD14 expression by leukocyte populations may differ in mice and humans. CD14 is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein). After binding to LPS-LPB, CD14 associates with integral membrane proteins (such as TLR4-MD-2) which mediate signal transduction. It is involved in the development of endotoxic shock, innate immunity, inflammatory responses, and LPS-stimulated bone resorption. Unlike mAb rmC5-3, the 4C1/CD14 antibody blocks the binding of LPS to CD14.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades