MAPK14 antibody (pThr180) (PerCP-Cy5.5)

Catalog No. ABIN1177119

Product Details anti-MAPK14 Antibody (PerCP-Cy5.5)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Binding Specificity: pThr180

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPK14 antibody is conjugated to PerCP-Cy5.5

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human p38 MAPK (pT180/pY182) Peptide

Clone: 36-p38

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

References for anti-MAPK14 antibody (PerCP-Cy5.5) (ABIN1177119)

Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, (1997) (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, (1996) (PubMed).

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, (1994) (PubMed).

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: p38 MAPK (MAPK14 Products)

Synonyms: CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, EXIP antibody, Mxi2 antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody, p38 antibody, p38ALPHA antibody, CRK1 antibody, Csbp1 antibody, Csbp2 antibody, Exip antibody, Hog antibody, Prkm14 antibody, Prkm15 antibody, Sapk2A antibody, p38Hog antibody, p38alpha antibody, p38b antibody, zp38b antibody, MAPK14 antibody, 186F5S antibody, BG:DS00797.3 antibody, CG7393 antibody, D-p38 antibody, D-p38 MAPK antibody, D-p38b antibody, Dm p38b antibody, Dmel\\CG7393 antibody, Dmp38b antibody, Dp38 antibody, Dp38b antibody, ESTS:186F5S antibody, Mpk34C antibody, anon-sts23 antibody, dp38b antibody, p38 MAPK antibody, p38 beta antibody, p38B antibody, p38Kb antibody, p38beta antibody, Crk1 antibody, p38-alpha antibody, p38MAPK antibody, p38a antibody, csbp antibody, mapk14a antibody, mxi2 antibody, sapk2 antibody, sapk2a antibody, AP22.98 antibody, AP22_98 antibody, ATMPK14 antibody, mitogen-activated protein kinase 14 antibody, SAPK2a antibody, MAP kinase 14A antibody, MAP kinase p38a antibody, MAPK 14A antibody, fk28c03 antibody, hm:zeh1243 antibody, wu:fk28c03 antibody, zp38a antibody, P38C-CRK antibody, mitogen-activated protein kinase 14 antibody, mitogen activated protein kinase 14 antibody, mitogen-activated protein kinase 14b antibody, p38b MAP kinase antibody, mitogen-activated protein kinase 14 S homeolog antibody, mitogen-activated protein kinase 14a antibody, CRK proto-oncogene, adaptor protein antibody, MAPK14 antibody, Mapk14 antibody, mapk14b antibody, p38b antibody, mapk14.S antibody, MPK14 antibody, mapk14a antibody, CRK antibody

Background: Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38alpha (MAPK14), beta (MAPK11), gamma (MAPK12), and delta (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways. The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38alpha, beta, gamma, and delta.
Synonyms: MK14, 11, 12, 13, CSBP1, SAPK2, 2A, 3, 4, MX12, ERK-6, ERK5

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins