PDGFRB antibody (pTyr857) (Alexa Fluor 488)

Catalog No. ABIN1177131

Product Details anti-PDGFRB Antibody (Alexa Fluor 488)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Binding Specificity: pTyr857

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRB antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PDGFRbeta Peptide

Clone: J24-618

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-PDGFRB antibody (Alexa Fluor 488) (ABIN1177131)

Liu, Oh, Horner, Rogers, Schnitzer: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, (1997) (PubMed).

Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, (1995) (PubMed).

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: CD140b (PDGFRB Products)

Synonyms: AI528809 antibody, CD140b antibody, PDGFR-1 antibody, Pdgfr antibody, CD140B antibody, IBGC4 antibody, JTK12 antibody, PDGFR antibody, PDGFR1 antibody, platelet derived growth factor receptor, beta polypeptide antibody, platelet derived growth factor receptor beta antibody, Pdgfrb antibody, PDGFRB antibody

Background: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma. The J24-618 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity. The orthologous phosphorylation site in mouse PDGFRbeta is Y856.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling