PKC alpha antibody (Protein Kinase C, alpha) (pThr497) (PE)

Details for Product anti-PKCa Antibody No. ABIN1177143, Supplier: Log in to see
Antigen
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Alternatives
anti-Human PKC alpha antibody for Immunohistochemistry (Frozen Sections)
Epitope
pThr497
38
19
18
17
17
15
15
15
11
7
7
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
300
214
171
31
26
25
21
21
20
5
3
3
2
1
1
1
1
Host
Mouse
268
45
2
Clonality (Clone)
Monoclonal ()
Conjugate
This PKC alpha antibody is conjugated to PE
14
13
10
6
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
257
143
134
71
55
45
34
26
26
23
13
4
4
4
2
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Phosflow™
Immunogen Phosphorylated Human PKCalpha Peptide
Clone K14-984
Isotype IgG1, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others PKC alpha products on genomics-online (e.g. as negative or positive controls)
Alternative Name PKCa (PKCa Antibody Abstract)
Background The Protein Kinase C (PKC) family of serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC). All have C-terminal kinase domains, which are closely related to those of protein kinases A and B (Akt), and variable N-terminal regulatory domains that give them different modes of activation. For example, cPKC (a, bI, bII, and g isoforms) are calcium-activated, phospholipid-dependent serine/threonine-specific enzymes that can also be activated by phorbol esters. However, nPKC (d, e, h, and q isoforms) and aPKC (z, i, and l isoforms) are Ca2+-independent. aPKC are unique in that their activity is independent of diacylglycerols and phorbol esters. Phosphorylation at three conserved sites in the kinase domain is required for catalytic activity. Specifically, the threonine 497 (T497) of PKCa is in the activation loop of the kinase domain and is phosphorylated by the constitutively active phosphoinositide-dependent kinase-1 (PDK-1). The K14-984 monoclonal antibody recognizes the phosphorylated T497 in the kinase domain of human PKCa.
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product cited in: Newton: "Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm." in: The Biochemical journal, Vol. 370, Issue Pt 2, pp. 361-71, 2003 (PubMed).

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