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STAT1 antibody (pSer727) (Alexa Fluor 647)

STAT1 Reactivity: Human, Mouse ICS Host: Mouse Monoclonal K51-856 Alexa Fluor 647
Catalog No. ABIN1177187
  • Target See all STAT1 Antibodies
    STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
    Binding Specificity
    • 51
    • 38
    • 16
    • 15
    • 13
    • 7
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer727
    Reactivity
    • 191
    • 103
    • 75
    • 18
    • 18
    • 16
    • 15
    • 13
    • 11
    • 7
    • 7
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 188
    • 21
    • 2
    Mouse
    Clonality
    • 165
    • 46
    Monoclonal
    Conjugate
    • 118
    • 11
    • 11
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    This STAT1 antibody is conjugated to Alexa Fluor 647
    Application
    • 142
    • 67
    • 52
    • 47
    • 39
    • 32
    • 26
    • 26
    • 21
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human Stat1 Peptide
    Clone
    K51-856
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product STAT1 Primary Antibody
  • Application Notes
    Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation. Any of the three BD Phosflow™ permeabilization buffers may be used.
    Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Townsend, Scarabelli, Davidson, Knight, Latchman, Stephanou: "STAT-1 interacts with p53 to enhance DNA damage-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5811-20, (2004) (PubMed).

    Varinou, Ramsauer, Karaghiosoff, Kolbe, Pfeffer, Müller, Decker: "Phosphorylation of the Stat1 transactivation domain is required for full-fledged IFN-gamma-dependent innate immunity." in: Immunity, Vol. 19, Issue 6, pp. 793-802, (2003) (PubMed).

  • Target
    STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
    Alternative Name
    Stat1 (STAT1 Products)
    Synonyms
    CANDF7 antibody, ISGF-3 antibody, STAT91 antibody, 2010005J02Rik antibody, AA408197 antibody, XStat1 antibody, STAT4 antibody, DD6G4-4 antibody, STAT antibody, STAT1 antibody, dZ182H3.3 antibody, si:by134g18.2 antibody, si:by51f19.1 antibody, si:dz182h3.3 antibody, si:dz199m19.1 antibody, si:dz73o4.2 antibody, stat1 antibody, signal transducer and activator of transcription 1 antibody, signal transducer and activator of transcription 1 L homeolog antibody, signal transducer and activator of transcription 1, 91kDa antibody, signal transducer and activator of transcription 4 antibody, signal transducer and activator of transcription 1a antibody, STAT1 antibody, Stat1 antibody, stat1.L antibody, stat4 antibody, stat1a antibody
    Background
    Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Furthermore, phosphorylation of serine 727 (S727) of Stat1 may occur in response to pathogens, cytokines, UV irradiation, and engagement of T and B cell antigen receptors. This second phosphorylation event is necessary for fully effective activation of transcription and may differentially regulate gene activation by interacting with other regulatory factors, such as p53 in the apoptotic pathway. The K51-856 monoclonal antibody recognizes the phosphorylated S727 in the C-terminal transactivating domain of human Stat1alpha, this site is not present in Stat1beta.
    Pathways
    JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
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