STAT6 antibody (pTyr641) (Alexa Fluor 488)

Catalog No. ABIN1177232

Product Details anti-STAT6 Antibody (Alexa Fluor 488)

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Binding Specificity: pTyr641

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT6 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Mouse STAT6 (Y641) Peptide

Clone: J71-773-58-11

Isotype: IgG1 kappa

Application Details

Application Notes: For flow cytometric analysis of human cells, we recommend Alexa Fluor 488 Mouse anti-Stat6 (pY641) mAb 18 (Cat. no. 612600).

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-STAT6 antibody (Alexa Fluor 488) (ABIN1177232)

Hale, Krutzik, Samra, Crane, Nolan: "Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus." in: PLoS ONE, Vol. 4, Issue 8, pp. e6756, (2009) (PubMed).

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

Target Details for STAT6

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Alternative Name: Stat6 (STAT6 Products)

Synonyms: D12S1644 antibody, IL-4-STAT antibody, STAT6B antibody, STAT6C antibody, signal transducer and activator of transcription 6 antibody, signal transducer and activator of transcription 6-like antibody, STAT6 antibody, Stat6 antibody, LOC100859543 antibody

Background: STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. The J71-773.58.11 antibody recognizes mouse Stat6 phosphorylated at Y641.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response