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ERK1/2 antibody (pThr202) (Alexa Fluor 488)

MAPK1/3 Reactivity: Human, Mouse, Rat ICS Host: Mouse Monoclonal 20A Alexa Fluor 488
Catalog No. ABIN1177245
  • Target See all ERK1/2 (MAPK1/3) Antibodies
    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
    Binding Specificity
    • 49
    • 41
    • 30
    • 30
    • 16
    • 12
    • 12
    • 10
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr202
    Reactivity
    • 193
    • 163
    • 160
    • 23
    • 23
    • 18
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 144
    • 51
    • 4
    Mouse
    Clonality
    • 140
    • 59
    Monoclonal
    Conjugate
    • 98
    • 12
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    This ERK1/2 antibody is conjugated to Alexa Fluor 488
    Application
    • 156
    • 65
    • 51
    • 51
    • 50
    • 38
    • 25
    • 23
    • 22
    • 13
    • 4
    • 4
    • 3
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Rat ERK1 (T202/Y204) Peptide
    Clone
    20A
    Isotype
    IgG1
    Top Product
    Discover our top product MAPK1/3 Primary Antibody
  • Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
  • Irish, Czerwinski, Nolan, Levy: "Altered B-cell receptor signaling kinetics distinguish human follicular lymphoma B cells from tumor-infiltrating nonmalignant B cells." in: Blood, Vol. 108, Issue 9, pp. 3135-42, (2006) (PubMed).

    Irish, Czerwinski, Nolan, Levy: "Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 3, pp. 1581-9, (2006) (PubMed).

    Krutzik, Nolan: "Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling." in: Nature methods, Vol. 3, Issue 5, pp. 361-8, (2006) (PubMed).

    Clark, Hynes: "Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14814-8, (1996) (PubMed).

    Boulton, Cobb: "Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies." in: Cell regulation, Vol. 2, Issue 5, pp. 357-71, (1991) (PubMed).

  • Target
    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
    Alternative Name
    ERK1/2 (MAPK1/3 Products)
    Synonyms
    erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
    Background
    The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The 20A monoclonal antibody recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and pT184/pY186 of human ERK2. The orthologous phosphorylation sites in murine ERK1 and ERK2 are T203/Y205 and T183/Y185.
    Synonyms: p44/42 MAPK, Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
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