TNF alpha antibody

Catalog No. ABIN1177286

Product Details anti-TNF alpha Antibody

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 2.0 ng/mL recombinant human TNF (Cat. No. 554618) and L929 at 2 x 10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human TNF for 60 minutes).
50% Neutralization (ND50) at 1 -100 ng/ml
>90% Neutralization at 50-300 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant human TNF protein

Clone: MABTNF-A5

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-TNF alpha antibody (ABIN1177286)

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." in: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).

Kriegler, Perez, DeFay, Albert, Lu: "A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF." in: Cell, Vol. 53, Issue 1, pp. 45-53, (1988) (PubMed).

Smith, Baglioni: "The active form of tumor necrosis factor is a trimer." in: The Journal of biological chemistry, Vol. 262, Issue 15, pp. 6951-4, (1987) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF (TNF alpha Products)

Synonyms: DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody

Background: The MABTNF-A5 antibody, formerly known as 5N, reacts with human tumor necrosis factor, TNF (aka, TNF-alpha and TNFSF2). TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer. The immunogen used to generate the MABTNF-A5 hybridoma was purified recombinant human TNF. This is an extraordinarily potent neutralizing antibody that is reportedly also useful for ELISA measurements of human TNF.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome