FAS antibody

Catalog No. ABIN1177304

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: WR19L mouse lymphoma cells transformed with recombinant mouse Fas

Clone: Jo2

Isotype: IgG2 lambda

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-FAS antibody (ABIN1177304)

Enari, Hug, Nagata: "Involvement of an ICE-like protease in Fas-mediated apoptosis." in: Nature, Vol. 375, Issue 6526, pp. 78-81, (1995) (PubMed).

Nagata: "Apoptosis regulated by a death factor and its receptor: Fas ligand and Fas." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 345, Issue 1313, pp. 281-7, (1995) (PubMed).

Nagata: "Fas and Fas ligand: a death factor and its receptor." in: Advances in immunology, Vol. 57, pp. 129-44, (1995) (PubMed).

Ni, Tomita, Matsuda, Ichihara, Ishimura, Ogasawara, Nagata: "Fas-mediated apoptosis in primary cultured mouse hepatocytes." in: Experimental cell research, Vol. 215, Issue 2, pp. 332-7, (1995) (PubMed).

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." in: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." in: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." in: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Background: Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb has been reported to immunoprecipitate mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation.
Synonyms: Fas/APO-1

Molecular Weight: 45 kDa

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity