No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative
Azide free
Storage
4 °C
Storage Comment
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Groh, Bruhl, El-Gabalawy, Nelson, Spies: "Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9452-7, (2003) (PubMed).
Groh, Rhinehart, Randolph-Habecker, Topp, Riddell, Spies: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, (2001) (PubMed).
Roberts, Lee, Schwarz, Groh, Spies, Ebert, Jabri: "NKG2D receptors induced by IL-15 costimulate CD28-negative effector CTL in the tissue microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5527-30, (2001) (PubMed).
Steinle, Li, Morris, Groh, Lanier, Strong, Spies: "Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family." in: Immunogenetics, Vol. 53, Issue 4, pp. 279-87, (2001) (PubMed).
Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, (1999) (PubMed).
Target
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1. NKG2D is a member of the C-type lectin family and is expressed on human NK cells. This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types. Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells. It is also expressed on virtually all TCR gamma/delta+ and CD8+TCR alpha/beta+ T cells. NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells. Importantly, NKG2D can complement the role of NCR in tumor cell lysis. Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells. The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells. Synonyms: CD314, KLRK1, KLR, NKG2D, NKG2-D, NK cell receptor D