LY6C + LY6G antibody

Catalog No. ABIN1177321

Product Details

Target: LY6C + LY6G

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: RB6-8C5

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

Tumpey, Chen, Oakes, Lausch: "Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea." in: Journal of virology, Vol. 70, Issue 2, pp. 898-904, (1996) (PubMed).

Rakhmilevich: "Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes." in: Journal of leukocyte biology, Vol. 57, Issue 6, pp. 827-31, (1995) (PubMed).

Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).

Czuprynski, Brown, Maroushek, Wagner, Steinberg: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, (1994) (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).

Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." in: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).

Target Details

Target: LY6C + LY6G

Alternative Name: Ly-6G, Ly-6C

Background: The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the lineage cocktail used in studies of hematopoietic lineages. The mAb 1A8 (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. Based on the comparison of the staining patterns of mAbs clones 1A8 and RB6-8C5 on total blood leukocytes, it is evident that mAb 1A8 stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes, whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the TDS Cat. No. 551459 and 553128 for more detail information.
Synonyms: Gr-1